Difference between revisions of "Part:BBa K4147005"
 
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This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).
 
This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).
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===Usage and Biology===
  
 
This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as <i>Alternaria</i>, <i>Cercospora </i>, <i>Fusarium</i>, <i>Phytophthora</i>, <i>Pythium</i>, <i>Rhizoctonia</i> and <i>Verticillium</i> sp [1].
 
This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as <i>Alternaria</i>, <i>Cercospora </i>, <i>Fusarium</i>, <i>Phytophthora</i>, <i>Pythium</i>, <i>Rhizoctonia</i> and <i>Verticillium</i> sp [1].
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4147005 SequenceAndFeatures</partinfo>
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==References==
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[1] Osusky, M., Osuska, L., Kay, W., & Misra, S. (2005). Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2. Theoretical and Applied Genetics, 111(4), 711–722. doi:10.1007/s00122-005-2056-y

Latest revision as of 09:38, 8 October 2022

Dermaseptin B1 (AKA MsrA2) expression construct with LacI regulated promoter

This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).

Usage and Biology

This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as Alternaria, Cercospora , Fusarium, Phytophthora, Pythium, Rhizoctonia and Verticillium sp [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Osusky, M., Osuska, L., Kay, W., & Misra, S. (2005). Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2. Theoretical and Applied Genetics, 111(4), 711–722. doi:10.1007/s00122-005-2056-y