Difference between revisions of "Part:BBa K4193011:Design"

 
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===Design Notes===
 
===Design Notes===
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quorum sensing system (two parts)
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we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription.
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+
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===Source===
 
===Source===
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synthesize from company
−
quorum sensing system (two parts)
+
  
 
===References===
 
===References===
 +
Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.

Revision as of 08:22, 8 October 2022


quorum sensing system (two parts)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription.

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.