Difference between revisions of "Part:BBa K4193021:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
ribozyme riboswitch 1-2L
+
By using a RNA-based gene-regulatory platform, we design a ribozyme switch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
ribozyme riboswitch 1-2L
+
synthesize from company
  
 
===References===
 
===References===
 +
Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.

Latest revision as of 07:34, 8 October 2022


ribozyme riboswitch 1-2L


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 61
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 61
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By using a RNA-based gene-regulatory platform, we design a ribozyme switch contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.


Source

synthesize from company

References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.