Difference between revisions of "Part:BBa K4137009:Design"
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https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | ||
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https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) | ||
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https://parts.igem.org/Part:BBa_K1659003 | https://parts.igem.org/Part:BBa_K1659003 | ||
===References=== | ===References=== | ||
Revision as of 07:21, 8 October 2022
T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1523
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1036
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 583
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.
Source
https://parts.igem.org/Part:BBa_K1659003