Difference between revisions of "Part:BBa K1054002"
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B.The ‘death raft’ model for holin lesion formation. (Mart Krupovič, Dennis H. Bamford. 2008) </p > | B.The ‘death raft’ model for holin lesion formation. (Mart Krupovič, Dennis H. Bamford. 2008) </p > | ||
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Revision as of 06:49, 8 October 2022
bacteriophage PBSX holin
Holin is an essential protein for host lysis by bacteriophage. This part is cloned from Bacillus subtilis subsp. subtilis str. 168. Its activity can by blocked by another protein called lrgA (BBa_K1054024) and lrgB (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid.
Growth curve of BL21 E.coli cells induced by different concentrations of IPTG
< img src="https://static.igem.wiki/teams/4144/wiki/contribution/part-bronze2.png" width="100%" height="100%">
Figure 1: A.The prediction of transmembrane domain of PBSX holin. B.The ‘death raft’ model for holin lesion formation. (Mart Krupovič, Dennis H. Bamford. 2008)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]