Difference between revisions of "Part:BBa K4268005:Experience"
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− | [[File: T-suny-oneonta-t7- | + | [[File: T-suny-oneonta-t7-tail tubular protein B colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Tail Tubular Protein B insert. The insert length is 2751bp and the predicted size of the PCR product when using VF/VR primers is 2917bp.]] |
− | The gel indicates that all five colonies are likely to contain the correct insert and thus, the | + | The gel indicates that all five colonies are likely to contain the correct insert and thus, the Tail Tubular Protein B was successfully cloned into a Level 0 Golden Gate Assembly basic part. |
===Applications of BBa_K4268005=== | ===Applications of BBa_K4268005=== | ||
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<I>Username</I> | <I>Username</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
− | Enter the review | + | Enter the review information here. |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K4268005 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K4268005 EndReviews</partinfo> |
Revision as of 06:27, 8 October 2022
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Tail Tubular Protein B was successfully cloned into a Level 0 Golden Gate Assembly basic part.
Applications of BBa_K4268005
User Reviews
UNIQ4b54a9e84c77e576-partinfo-00000000-QINU UNIQ4b54a9e84c77e576-partinfo-00000001-QINU