Difference between revisions of "Part:BBa K4199002"
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The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]
 
  
 
===Usage and Biology===
 
===Usage and Biology===
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The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]
  
 
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Revision as of 06:20, 8 October 2022


HrtR

The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]


Usage and Biology

The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 249
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]