Difference between revisions of "Part:BBa K4193002:Design"
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===Source=== | ===Source=== | ||
− | + | synthesize from company | |
===References=== | ===References=== | ||
+ | Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104. |
Revision as of 04:55, 8 October 2022
ribozyme riboswitch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 38
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 38
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 38
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 38
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.
![](https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg)
Source
synthesize from company
References
Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.