Difference between revisions of "Part:BBa K4137008"
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__NOTOC__
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<partinfo>BBa_K4137008 short</partinfo>
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This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.
 
This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4137008 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4137008 parameters</partinfo>
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Revision as of 03:11, 8 October 2022


mleR expressing construct

This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]