Difference between revisions of "Part:BBa K4146507"

Revision as of 03:07, 8 October 2022

pCS-aroGfbr

 aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study.

We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.

 Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.

          The standard curve for determination of 2-phenylethanol yield

          The experimental 2-phenylethanol yield

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal BamHI site found at 738
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4146507 short</partinfo>
-   aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
+   aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study.
+We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
    Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.