Difference between revisions of "Part:BBa K4146507"

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   aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
 
   aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
 
   Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.
 
   Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.
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[[File:The standard curve for determination of 2-phenylethanol yield.png|500px]]
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[[File:The experimental 2-phenylethanol yield.png|500px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:52, 8 October 2022


pCS-aroGfbr 

 aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
 Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.

The standard curve for determination of 2-phenylethanol yield.png

The experimental 2-phenylethanol yield.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 1000
    COMPATIBLE WITH RFC[1000]