Difference between revisions of "Part:BBa K4268004:Experience"
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Revision as of 00:11, 8 October 2022
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
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UNIQ79f2711bec653a3e-partinfo-00000000-QINU UNIQ79f2711bec653a3e-partinfo-00000001-QINU