Difference between revisions of "Part:BBa K4147002:Design"

Revision as of 00:08, 8 October 2022

DESIGN NOTES

It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.

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−	+	===DESIGN NOTES===
	+	It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.