Difference between revisions of "Part:BBa K4368000:Design"
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===Source=== | ===Source=== | ||
| − | The ''cenA'' gene is an optimized synthetic construction based on: <partinfo>BBa_K118023</partinfo> . | + | The ''cenA'' gene is an optimized synthetic construction based on: <partinfo>BBa_K118023</partinfo>. |
===References=== | ===References=== | ||
| + | * '''Gohel, H. R., Contractor, C. N., Ghosh, S. K., & Braganza, V. J.''' (2014). A comparative study of various staining techniques for determination of extra cellular cellulase activity on Carboxy Methyl Cellulose (CMC) agar plates. ''Int J Curr Microbiol App Sci, 3(5), 261-266. | ||
Latest revision as of 18:50, 7 October 2022
pcstA + rbs + yebF + cenA + terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 554
Illegal NotI site found at 1698 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BglII site found at 1611
Illegal BamHI site found at 747
Illegal XhoI site found at 1109
Illegal XhoI site found at 1358 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 889
Illegal NgoMIV site found at 1814 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 793
Design Notes
Consideration should be given to the correct optimization of the sequence for use in different organisms.
Source
The cenA gene is an optimized synthetic construction based on: BBa_K118023.
References
- Gohel, H. R., Contractor, C. N., Ghosh, S. K., & Braganza, V. J. (2014). A comparative study of various staining techniques for determination of extra cellular cellulase activity on Carboxy Methyl Cellulose (CMC) agar plates. Int J Curr Microbiol App Sci, 3(5), 261-266.