Difference between revisions of "Part:BBa K4180001"
 
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Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose
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Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose<br>
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Galactose promoter can be induced in the presence of galactose and suppressed in the presence of glucose<br><br>
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Citation: Investigations in Molecular Cell Biology (O'Connor);  13.1: Regulation of the GAL1 promoter;
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<https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.01%3A_Regulation_of_the_GAL1_promoter>
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<br><br>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<!--[[File:BBa K4180001.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;  BBa_K4180000 SNF1 full length <br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
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[[File:BBa K4180000-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]-->
  
  

Latest revision as of 17:34, 7 October 2022


pGal promoter

Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose
Galactose promoter can be induced in the presence of galactose and suppressed in the presence of glucose

Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter; <https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.01%3A_Regulation_of_the_GAL1_promoter>

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]