Difference between revisions of "Part:BBa K4268003"

Revision as of 15:27, 7 October 2022

S-TIP37 Capsid Protein with Biotin Tag

Usage and Biology

S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle (Shitrit et al., 2021). T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host (Raytcheva et al., 2011).

Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)

This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.

However, it will infect Synechococcus sp WH 8109, the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as Synechococcus sp PCC 11901.

This part combines the Capsid Protein gene (gp33) which has been provisionally identified, with a biotin tag to form the Capsid Protein with Biotin Tag part. This part codes for one of the proteins that are responsible for building the icosahedral capsid. To meet a need in the community for immobilizing cyanobacteria, this part was developed to have the addition of a biotin tag. The use of a biotin-streptavidin bond in our phage will immobilize the cyanobacteria that have been bonded to the phages.

Sequence and Features