Difference between revisions of "Part:BBa K4231017"
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Using pUC19 as plasmid backbone, AmpR as reporter gene in bacteria, URA3 tag as reporter gene in Yarrowia lipolytica, shuttle plasmid carrying ERG10 and ERG13 from Yarrowia lipolytica. The plasmid can be effectively amplified in E.coli DH5a and can be successfully expressed in Yarrowia lipolytica. At the same time, ERG10 and ERG13 are important enzyme genes in the MVA pathway, and they carry the PTS1 sequence to help the enzyme protein locate in the peroxisome. Therefore, the plasmid plays an important role in the construction of MVA pathway in the peroxisome of Yarrowia lipolytica. | Using pUC19 as plasmid backbone, AmpR as reporter gene in bacteria, URA3 tag as reporter gene in Yarrowia lipolytica, shuttle plasmid carrying ERG10 and ERG13 from Yarrowia lipolytica. The plasmid can be effectively amplified in E.coli DH5a and can be successfully expressed in Yarrowia lipolytica. At the same time, ERG10 and ERG13 are important enzyme genes in the MVA pathway, and they carry the PTS1 sequence to help the enzyme protein locate in the peroxisome. Therefore, the plasmid plays an important role in the construction of MVA pathway in the peroxisome of Yarrowia lipolytica. | ||
| + | [[Image:pUC-10-13-URA 图谱.png | border | center | 400px]] | ||
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Revision as of 12:10, 7 October 2022
pUC19 carrying with ERG10, ERG13 and URA3 tag from Y.lipolytica
Using pUC19 as plasmid backbone, AmpR as reporter gene in bacteria, URA3 tag as reporter gene in Yarrowia lipolytica, shuttle plasmid carrying ERG10 and ERG13 from Yarrowia lipolytica. The plasmid can be effectively amplified in E.coli DH5a and can be successfully expressed in Yarrowia lipolytica. At the same time, ERG10 and ERG13 are important enzyme genes in the MVA pathway, and they carry the PTS1 sequence to help the enzyme protein locate in the peroxisome. Therefore, the plasmid plays an important role in the construction of MVA pathway in the peroxisome of Yarrowia lipolytica.
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 634
Illegal PstI site found at 3745 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Unknown