Difference between revisions of "Part:BBa K4121020:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.
+
Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 11:18, 7 October 2022


The Acetylacetyl-CoA thiolytic enzyme gene ERG10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 958
    Illegal PstI site found at 773
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 958
    Illegal PstI site found at 773
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 958
    Illegal BglII site found at 697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 958
    Illegal PstI site found at 773
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 958
    Illegal PstI site found at 773
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.

Source

National Center for Biotechnology Information (NCBI)

References