Difference between revisions of "Part:BBa K4375016"

 
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<partinfo>BBa_K4375016 short</partinfo>
 
<partinfo>BBa_K4375016 short</partinfo>
  
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This construct is meant for detecting cancer cells that express EGFR receptors by bacterial cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). EGFR-positive cell-specific attachment is achieved by an anti-EGFR-specific Nanobody, 7D12, (BBa_K4375005). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorercence of the policistronicly expressed sfGFP.  
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This construction consists of 4 described parts. These are:  J23101* (BBa_K4375003), anti-EGFR-specific Nanobody: 7D12 (BBa_K4375005), Intimin (BBa_K4375012),  Myc (BBa_K4375014). They allow the bacterium to be expelled to the tumour surface, thus allowing its visualisation and colonisation on the tumour surface.
  
  
  
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==Usage and Biology==
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===Usage and Biology===
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This construct is meant for detecting cancer cells expressing EGFR receptors.This construct is expressed by the bacterial cells on their surface and can be used to detect the tumour by attaching to cancer cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). EGFR-positive cell-specific attachment is achieved by an anti-EGFR-specific Nanobody, 7D12, (BBa_K4375005). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorercence of the policistronicly expressed sfGFP.
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Revision as of 09:29, 7 October 2022


anti-EGFR Nanobody Display System

This construction consists of 4 described parts. These are: J23101* (BBa_K4375003), anti-EGFR-specific Nanobody: 7D12 (BBa_K4375005), Intimin (BBa_K4375012), Myc (BBa_K4375014). They allow the bacterium to be expelled to the tumour surface, thus allowing its visualisation and colonisation on the tumour surface.


Usage and Biology

This construct is meant for detecting cancer cells expressing EGFR receptors.This construct is expressed by the bacterial cells on their surface and can be used to detect the tumour by attaching to cancer cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). EGFR-positive cell-specific attachment is achieved by an anti-EGFR-specific Nanobody, 7D12, (BBa_K4375005). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorercence of the policistronicly expressed sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2029
    Illegal BamHI site found at 4045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2050
    Illegal NgoMIV site found at 4066
    Illegal AgeI site found at 4124
    Illegal AgeI site found at 4256
    Illegal AgeI site found at 4640
  • 1000
    COMPATIBLE WITH RFC[1000]