Difference between revisions of "Part:BBa K4375014"

 
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Myc Tag derived from c-Myc.
 
Myc Tag derived from c-Myc.
  
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==Usage and Biology==
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===Usage and Biology===
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The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor.
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A Myc-tag can be used to detect expression of recombinant proteins.
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First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4375014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4375014 SequenceAndFeatures</partinfo>
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==References==
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https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/
  
  

Revision as of 09:26, 7 October 2022


Myc Tag

Myc Tag derived from c-Myc.

Usage and Biology

The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor. A Myc-tag can be used to detect expression of recombinant proteins. First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/