Difference between revisions of "Part:BBa K4375013"

Revision as of 09:25, 7 October 2022

Myc epitope tag with SphI restriction site

A myc tag is a polypeptide protein tag derived from the c-myc gene, and we appended a SpHI restriction site to its N-terminal.

Usage and Biology

The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor. A Myc-tag can be used to detect expression of recombinant proteins. First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.

Sequence and Features

References

https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/