Difference between revisions of "Part:BBa K4375013"
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A myc tag is a polypeptide protein tag derived from the c-myc gene, and we appended a SpHI restriction site to its N-terminal. | A myc tag is a polypeptide protein tag derived from the c-myc gene, and we appended a SpHI restriction site to its N-terminal. | ||
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| − | + | ==Usage and Biology== | |
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| + | The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor. | ||
| + | A Myc-tag can be used to detect expression of recombinant proteins. | ||
| + | First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting. | ||
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Revision as of 09:23, 7 October 2022
Myc epitope tag with SphI restriction site
A myc tag is a polypeptide protein tag derived from the c-myc gene, and we appended a SpHI restriction site to its N-terminal.
Usage and Biology
The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor. A Myc-tag can be used to detect expression of recombinant proteins. First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]