Difference between revisions of "Part:BBa K4165209"

Revision as of 09:17, 7 October 2022

Coh2 linked with tau binding peptide (WINK) & G4S linker

Fusion protein consists of Tau binding peptide (WINK) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.

Usage and Biology

Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim , we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations . Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.

3D Modeling and ranking

Fused protein consists of WINK tau binding peptide (BBa_K4165146) with linker (GGGGS)3 (BBa_K4165068) to link between the binding peptide and Coh2 (BBa_K4165003). this model was designed by Modeller tool with QA 5 out of 6 according to our parameters in QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its decrease in the overall protein stability as compared to other models.

                                   Figure 1. Predicted 3D structure of CoH2-(G4S)3-WINK protein.

Sequence and Features