Difference between revisions of "Part:BBa K4375008"

 
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<partinfo>BBa_K4375008 short</partinfo>
 
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miRFP670nano is a near ir fluorescent protein published in 2019, derived from Nostoc punctiforme. It is special because it harbors an usual small size. (17&#8201;kDa)
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miRFP670nano is a near ir fluorescent protein published in 2019, derived from Nostoc punctiforme. It is special because it harbours an usual small size. (17 kDa)
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==Usage and Biology==
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miRFP670nano is a near-infrared fluorescent protein (NIR FP) with excitation at 645 nm and emission at 670 nm. Efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells. miRFP670nano is a monomer with molecular weight of 17 kDa that is 2-fold smaller than bacterial phytochrome (BphP)-based NIR FPs and 1.6-fold smaller than GFP-like fluorescent proteins. It is highly stable to denaturation and degradation and can be used as an internal protein tag
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K4375008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4375008 SequenceAndFeatures</partinfo>
  
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==References==
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https://www.nature.com/articles/s41467-018-08050-8
  
 
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Revision as of 09:14, 7 October 2022


miRFP670 nano

miRFP670nano is a near ir fluorescent protein published in 2019, derived from Nostoc punctiforme. It is special because it harbours an usual small size. (17 kDa)


Usage and Biology

miRFP670nano is a near-infrared fluorescent protein (NIR FP) with excitation at 645 nm and emission at 670 nm. Efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells. miRFP670nano is a monomer with molecular weight of 17 kDa that is 2-fold smaller than bacterial phytochrome (BphP)-based NIR FPs and 1.6-fold smaller than GFP-like fluorescent proteins. It is highly stable to denaturation and degradation and can be used as an internal protein tag


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]

References

https://www.nature.com/articles/s41467-018-08050-8