Difference between revisions of "Part:BBa K4165206"

 
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Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.
 
Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.
  
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===Usage and Biology===
 
===Usage and Biology===
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DOC - CoH system was used for many targeting biological applications . In our case we introduce a fusion protein with Tau binding peptide (M4W39) for targeting Tau  with different methods so aggregations inhibition can be further tested in vivo using this part.
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===3D Model Photo and ranking===
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This fused protein was modeled using RosettaFold software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6.
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This protein was excluded due to its low QA score as compared to other models
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmm.png" style="margin-left:200px;" alt="" width="500" /></p>
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                                      Figure 1. Predicted 3D structure of CoH2-(G4S)3-M4W39 protein.
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Revision as of 08:38, 7 October 2022


Coh2 linked with tau binding peptide (M4W39)

Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.


Usage and Biology

DOC - CoH system was used for many targeting biological applications . In our case we introduce a fusion protein with Tau binding peptide (M4W39) for targeting Tau with different methods so aggregations inhibition can be further tested in vivo using this part.

3D Model Photo and ranking

This fused protein was modeled using RosettaFold software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its low QA score as compared to other models 

                                      Figure 1. Predicted 3D structure of CoH2-(G4S)3-M4W39 protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]