Difference between revisions of "Part:BBa K4192010"
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The cdg (BBa_K4192010) encodes the DGC protein of Pseudomonas fluorescens,also named as dgc. DGC protein catalyzes the synthesis of r c-di-GMP,which is an important regulator<sup>[1]</sup>. It can promote bacterial adhesion, extracellular polysaccharide synthesis and other functions.
 
The cdg (BBa_K4192010) encodes the DGC protein of Pseudomonas fluorescens,also named as dgc. DGC protein catalyzes the synthesis of r c-di-GMP,which is an important regulator<sup>[1]</sup>. It can promote bacterial adhesion, extracellular polysaccharide synthesis and other functions.
 
Increasing its expression can increase the production of biofilm.  
 
Increasing its expression can increase the production of biofilm.  
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 +
===Characterization===
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<p>We increased the expression number of <i>cdg</i> gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production</p>
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<p>We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see https://parts.igem.org/Part:BBa_K4192114.</p>
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Revision as of 07:19, 7 October 2022


cdg gene from Pseudomonas fluorescens str.2P24

The cdg (BBa_K4192010) encodes the DGC protein of Pseudomonas fluorescens,also named as dgc. DGC protein catalyzes the synthesis of r c-di-GMP,which is an important regulator[1]. It can promote bacterial adhesion, extracellular polysaccharide synthesis and other functions. Increasing its expression can increase the production of biofilm.

Characterization

We increased the expression number of cdg gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production

We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see https://parts.igem.org/Part:BBa_K4192114.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BamHI site found at 254
    Illegal XhoI site found at 352
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 187
    Illegal AgeI site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 772



References

[1]Ivanov, Ivan E et al. “Atomic force and super-resolution microscopy support a role for LapA as a cell-surface biofilm adhesin of Pseudomonas fluorescens.” Research in microbiology vol. 163,9-10 (2012): 685-91. doi:10.1016/j.resmic.2012.10.001