Difference between revisions of "Part:BBa K4192011"
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===Characterization===
 
===Characterization===
 
<p>We increased the expression number of <i>cdg</i> gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production</p>
 
<p>We increased the expression number of <i>cdg</i> gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production</p>
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<p>We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see <a>https://parts.igem.org/Part:BBa_K4192114</a>.
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<p>We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see https://parts.igem.org/Part:BBa_K4192114.
  
  
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===References===
 
===References===
 
<p>[1]Seaton, Sarah C et al. “Pleiotropic effects of GacA on Pseudomonas fluorescens Pf0-1 in vitro and in soil.” Applied and environmental microbiology vol. 79,17 (2013): 5405-10. doi:10.1128/AEM.00819-13</p>
 
<p>[1]Seaton, Sarah C et al. “Pleiotropic effects of GacA on Pseudomonas fluorescens Pf0-1 in vitro and in soil.” Applied and environmental microbiology vol. 79,17 (2013): 5405-10. doi:10.1128/AEM.00819-13</p>

Revision as of 07:04, 7 October 2022


gacA from Pseudomonas fluorescens str. 2P24

It encoding global regulator GacA. It can enhance extracellular polysaccharide (EPS) synthesis, enhance antifungal ability and weaken exercise ability[1]. Therefore, when it is co-expressed with other regulatory genes, the production of biofilm can be significantly increased. It belongs to a two-component regulatory system, typically composed of a membrane-bound sensor kinase (GacS) and a cytoplasmic response protein (GacA), distribute widely in bacteria. A number of publications demonstrated that the GacS/GacA system globally regulated the production of the extracellular compounds and exoenzymes and played an important role in the biocontrol activity of the Pseudomonas spp[2]. GacA can regulate a variety of downstream physiological activities, so the effect is not single. It even increase the content of c-di-GMP, similar to the cdg gene (BBa_K4192010).

Characterization

We increased the expression number of cdg gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production

We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see https://parts.igem.org/Part:BBa_K4192114. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<p>[1]Seaton, Sarah C et al. “Pleiotropic effects of GacA on Pseudomonas fluorescens Pf0-1 in vitro and in soil.” Applied and environmental microbiology vol. 79,17 (2013): 5405-10. doi:10.1128/AEM.00819-13

[2]Yan Xiaoxue.The role of regulatory gene gacA in the biocontrol activity of Pseudomonas fluorescens 2P24.2004.Northwest Sci-Tech University of Agriculture and Forestry,PhD dissertation..