Difference between revisions of "Part:BBa K4298012"

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<partinfo>BBa_K4298012 short</partinfo>
 
<partinfo>BBa_K4298012 short</partinfo>
  
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PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.
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pUC19 plasmid, containing flanked inverted repeats and the insertion sequence is EPI with α-secretion signal. EPI between the inverted repeat can be introduced into P1 plasmid by homologous recombination at both ends.
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The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid
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Latest revision as of 06:11, 7 October 2022


pUC19-ITR-EPI

pUC19 plasmid, containing flanked inverted repeats and the insertion sequence is EPI with α-secretion signal. EPI between the inverted repeat can be introduced into P1 plasmid by homologous recombination at both ends.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1000
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505