Difference between revisions of "Part:BBa K4368001:Design"
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===Design Notes===
 
===Design Notes===
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Consideration should be given to the correct optimization of the sequence for use in different organisms.
  
  

Revision as of 22:52, 6 October 2022


pcstA + rbs + yebF + cex + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1050
    Illegal NgoMIV site found at 1552
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097
    Illegal SapI.rc site found at 1180


Design Notes

Consideration should be given to the correct optimization of the sequence for use in different organisms.


Source

The cex gene is from Cellulomonas fimi, while the remaining parts are from Escherichia coli. The whole construct has been optimised for use in E. coli.

References