Difference between revisions of "Part:BBa K4368000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Consideration should be given to the correct | + | Consideration should be given to the correct optimization of the sequence for use in different organisms. |
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===Source=== | ===Source=== | ||
− | The cenA gene is from Cellulomonas fimi, while the remaining parts are from Escherichia coli. The whole construct has been optimised for use in E. coli. | + | The ''cenA'' gene is from ''Cellulomonas fimi'', while the remaining parts are from ''Escherichia coli''. The whole construct has been optimised for use in ''E. coli''. |
===References=== | ===References=== |
Revision as of 22:51, 6 October 2022
pcstA + rbs + yebF + cenA + terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 554
Illegal NotI site found at 1698 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BglII site found at 1611
Illegal BamHI site found at 747
Illegal XhoI site found at 1109
Illegal XhoI site found at 1358 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 889
Illegal NgoMIV site found at 1814 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 793
Design Notes
Consideration should be given to the correct optimization of the sequence for use in different organisms.
Source
The cenA gene is from Cellulomonas fimi, while the remaining parts are from Escherichia coli. The whole construct has been optimised for use in E. coli.