Difference between revisions of "Part:BBa K4165109"

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                   Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pyomol
 
                   Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pyomol
 
===Docking===
 
 
  
 
===References===
 
===References===

Revision as of 21:02, 6 October 2022


HTRA Binding Peptide 15

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with G of 10.3 ± 0.2 kcal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 17.86, molprobity = 4, ramachandran_favored = 62.5, ramachandran_outliers = 12.5, Qmean_4 = -6.45183, Qmean6 = -5.513969


Modeling


                 Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pyomol

References

Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119.