Difference between revisions of "Part:BBa K4165104"

 
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part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
 
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
  
 
 
 
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===Usage and Biology===
 
===Usage and Biology===
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Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –7.2 and IC50 of 16 ± 3
  
 
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===Functional Parameters===
 
===Functional Parameters===
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Got score of 3 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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cbeta_deviations = 0, clashscore = 0, molprobity = 1.52, ramachandran_favored = 40, ramachandran_outliers = 20, Qmean_4 = -3.013046, Qmean6 = -1.495219
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===Modeling===
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1j.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: 3D Model of H1J peptide Modelled by AlphaFold2.0 displayed in Pyomol
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<partinfo>BBa_K4165104 parameters</partinfo>
 
<partinfo>BBa_K4165104 parameters</partinfo>
 
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Revision as of 20:27, 6 October 2022


HTRA Binding Peptide 10

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –7.2 and IC50 of 16 ± 3

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Got score of 3 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 0, molprobity = 1.52, ramachandran_favored = 40, ramachandran_outliers = 20, Qmean_4 = -3.013046, Qmean6 = -1.495219

Modeling


                 Figure 1.: 3D Model of H1J peptide Modelled by AlphaFold2.0 displayed in Pyomol