Difference between revisions of "Part:BBa K4245002:Design"

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===Design Notes===
 
===Design Notes===
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The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (Autour et. al, 2016).
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This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245003, a composite part producing iSpinach-D5-G30-A32; the only difference being the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.
  
 
===Source===
 
===Source===

Revision as of 20:24, 6 October 2022


iSpinach aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 375
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245003, a composite part producing iSpinach-D5-G30-A32; the only difference being the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339