Difference between revisions of "Part:BBa J428112:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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Lambert iGEM transformed the InterLab Experiment 1 Test Device 1 (BBa_J428112) into two different strains of E. coli (BL21 and DH5-alpha) to characterize the differences in fluorescence. BL21 is used for protein expression, whereas DH5-alpha is utilized for plasmid transformation. Therefore, we expect the test device transformed into the BL21 cells to produce more fluorescence than those transformed into DH5-alpha. Lambert iGEM quantified the fluorescence at a 0 and 5 hour time point to determine the changes in fluorescence over a longer period of time. We used the iGEM InterLab Experiment 1 Protocol with minor alterations to characterize this part. The InterLab study instructs to quantify the sample at a 0 hour and 6 hour time mark. To better align with members’ schedules, we quantified fluorescence at a 0 hour and 5 hour time mark. Additionally, rather than quantifying 8 different test devices, we quantified only one in both BL21 and DH5-alpha to focus on the variance in fluorescence in different cell strains. | Lambert iGEM transformed the InterLab Experiment 1 Test Device 1 (BBa_J428112) into two different strains of E. coli (BL21 and DH5-alpha) to characterize the differences in fluorescence. BL21 is used for protein expression, whereas DH5-alpha is utilized for plasmid transformation. Therefore, we expect the test device transformed into the BL21 cells to produce more fluorescence than those transformed into DH5-alpha. Lambert iGEM quantified the fluorescence at a 0 and 5 hour time point to determine the changes in fluorescence over a longer period of time. We used the iGEM InterLab Experiment 1 Protocol with minor alterations to characterize this part. The InterLab study instructs to quantify the sample at a 0 hour and 6 hour time mark. To better align with members’ schedules, we quantified fluorescence at a 0 hour and 5 hour time mark. Additionally, rather than quantifying 8 different test devices, we quantified only one in both BL21 and DH5-alpha to focus on the variance in fluorescence in different cell strains. | ||
Revision as of 19:53, 6 October 2022
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how you used this part and how it worked out.
Characterization of BBa_J428112
Lambert iGEM transformed the InterLab Experiment 1 Test Device 1 (BBa_J428112) into two different strains of E. coli (BL21 and DH5-alpha) to characterize the differences in fluorescence. BL21 is used for protein expression, whereas DH5-alpha is utilized for plasmid transformation. Therefore, we expect the test device transformed into the BL21 cells to produce more fluorescence than those transformed into DH5-alpha. Lambert iGEM quantified the fluorescence at a 0 and 5 hour time point to determine the changes in fluorescence over a longer period of time. We used the iGEM InterLab Experiment 1 Protocol with minor alterations to characterize this part. The InterLab study instructs to quantify the sample at a 0 hour and 6 hour time mark. To better align with members’ schedules, we quantified fluorescence at a 0 hour and 5 hour time mark. Additionally, rather than quantifying 8 different test devices, we quantified only one in both BL21 and DH5-alpha to focus on the variance in fluorescence in different cell strains.
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