Difference between revisions of "Part:BBa K4239001"

Revision as of 19:31, 6 October 2022

Enhanced luciferase subunits fiatluxC

fiatluxC is made to be used with fiatluxD and fiatluxE. It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.

The systeme fiatluxC/fiatluxD/fiatluxE is made to be used with fiatluxA and fiatluxB, gathered in the fiatluxCDABE operon.

Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.

The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1311

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4239001 short</partinfo>
-fiatluxC is made to be used with  fiatluxD and fiatluxE. It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes.
+fiatluxC is made to be used with  fiatluxD and fiatluxE. It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.
-Luciferase has as substrats FMNH2, O2 and Fatty aldehydes, and produces H20, Fatty Acids and FMN and emits luminescence.
+The systeme fiatluxC/fiatluxD/fiatluxE is made to be used with fiatluxA and fiatluxB, gathered in the fiatluxCDABE operon.
-The systeme fiatluxC/fiatluxD is made to be used with fiatluxA, fiatluxB and fiatluxE, gathered in the fiatluxCDABE operon.
 Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.