Difference between revisions of "Part:BBa K4245002:Design"

 
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===Design Notes===
 
===Design Notes===
iSpinach aptamer with LacI repression
+
The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (https://academic.oup.com/nar/article/44/6/2491/2499459).
 
+
  
  
 
===Source===
 
===Source===
  
iSpinach aptamer with LacI repression
+
The part was acquired as synthesized sequences from IDT and Twist Bioscience.
  
 
===References===
 
===References===
 +
Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083
 +
 +
Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482
 +
 +
Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339

Revision as of 18:41, 6 October 2022


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Status: 500 Content-type: text/html

Software error:

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Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Design Notes

The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (https://academic.oup.com/nar/article/44/6/2491/2499459).


Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339