Difference between revisions of "Part:BBa K4494196"

 
 
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<partinfo>BBa_K4494196 short</partinfo>
 
<partinfo>BBa_K4494196 short</partinfo>
  
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This part consists of PIII (K4494002) and an RBS (B0034) combined using Golden Gate Assembly and works with A. caldus csoR (K4494001). Its variants are parts K4494003-K4494098.
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Note: This part does NOT work. For a working part please check our collection of variants K4494003-K4494098.
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==The K4494003-K4494098 Mutant Collection==
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Parts K4494003-K4494098 are mutants of K4494196 generated and characterized via high-throughput experimentation. For more information regarding this collection please check out [https://2022.igem.wiki/dku-china/part-collection our wiki.]The following shows some important statistical data regarding the collection.
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===Characterization===
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[[File:Heatmap.png|400px|thumb|center|Heatmap Characterization]]
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===Phylogeny===
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The following is the minimum-evolution tree of our collection (the numbers are variant order codes):
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[[File:Phylo.png|400px|thumb|center|Mimimum-Evolution Tree]]
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===Scatter Plot===
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The following scatter plot encompasses the foldchange, blank RFU, and Levenshtein Distance to K4494196:
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[[File:DKU.png|400px|thumb|center|Scatter Plot]]
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===Base Pair Count===
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[[File:Without1.png|600px|thumb|center|Unweighted Base Pair Frequency by Position]]
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[[File:Without2.png|600px|thumb|center|Unweighted Base Pair Percentages by Position]]
  
 
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Latest revision as of 15:27, 6 October 2022


Promoter-RBS Combination for A. caldus csoR

This part consists of PIII (K4494002) and an RBS (B0034) combined using Golden Gate Assembly and works with A. caldus csoR (K4494001). Its variants are parts K4494003-K4494098.

Note: This part does NOT work. For a working part please check our collection of variants K4494003-K4494098.

The K4494003-K4494098 Mutant Collection

Parts K4494003-K4494098 are mutants of K4494196 generated and characterized via high-throughput experimentation. For more information regarding this collection please check out our wiki.The following shows some important statistical data regarding the collection.

Characterization

Heatmap Characterization

Phylogeny

The following is the minimum-evolution tree of our collection (the numbers are variant order codes):

Mimimum-Evolution Tree

Scatter Plot

The following scatter plot encompasses the foldchange, blank RFU, and Levenshtein Distance to K4494196:

Scatter Plot

Base Pair Count

Unweighted Base Pair Frequency by Position
Unweighted Base Pair Percentages by Position

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]