Difference between revisions of "Part:BBa K4207023"

Revision as of 15:21, 6 October 2022

PMV toehold switch A2

Toehold switch for the detection of PMV gRNA

1. Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

2. Design

3. Characterization

Expected structures
Structure of inactive form
Structure of active form

@@ Line 4: / Line 4: @@
 Toehold switch for the detection of PMV gRNA
+===1. Usage and Biology===
-<!-- Add more about the biology of this part here
+<html>
-===Usage and Biology===
+<center>
+<div style="width: 60%;">
+<svg id="eOrKQUqjyvE1" viewBox="0 0 455 215" shape-rendering="geometricPrecision" text-rendering="geometricPrecision">
+<style><![CDATA[
+#eOrKQUqjyvE6_to {animation: eOrKQUqjyvE6_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE6_to__to { 0% {transform: translate(230px,115px)} 11.222222% {transform: translate(230px,115px)} 55.666667% {transform: translate(317.272727px,195px)} 72.333333% {transform: translate(390px,195px)} 100% {transform: translate(390px,195px)}} #eOrKQUqjyvE7_to {animation: eOrKQUqjyvE7_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE7_to__to { 0% {transform: translate(230px,125px)} 11.222222% {transform: translate(230px,125px)} 50.111111% {transform: translate(306.363636px,195px)} 72.333333% {transform: translate(400px,195px)} 100% {transform: translate(400px,195px)}} #eOrKQUqjyvE8_to {animation: eOrKQUqjyvE8_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE8_to__to { 0% {transform: translate(230px,135px)} 11.222222% {transform: translate(230px,135px)} 44.444444% {transform: translate(295.454545px,195px)} 72.222222% {transform: translate(410px,195px)} 100% {transform: translate(410px,195px)}} #eOrKQUqjyvE9_to {animation: eOrKQUqjyvE9_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE9_to__to { 0% {transform: translate(230px,145px)} 11.222222% {transform: translate(230px,145px)} 39% {transform: translate(284.545454px,195px)} 72.333333% {transform: translate(420px,195px)} 100% {transform: translate(420px,195px)}} #eOrKQUqjyvE10_to {animation: eOrKQUqjyvE10_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE10_to__to { 0% {transform: translate(230px,155px)} 11.222222% {transform: translate(230px,155px)} 33.444444% {transform: translate(273.636364px,195px)} 72.333333% {transform: translate(430px,195px)} 100% {transform: translate(430px,195px)}} #eOrKQUqjyvE11_to {animation: eOrKQUqjyvE11_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE11_to__to { 0% {transform: translate(230px,165px)} 11.222222% {transform: translate(230px,165px)} 27.888889% {transform: translate(262.727273px,195px)} 72.333333% {transform: translate(440px,195px)} 100% {transform: translate(440px,195px)}} #eOrKQUqjyvE12_to {animation: eOrKQUqjyvE12_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE12_to__to { 0% {transform: translate(230px,175px)} 11.222222% {transform: translate(230px,175px)} 22.333333% {transform: translate(251.818182px,195px)} 72.333333% {transform: translate(450px,195px)} 100% {transform: translate(450px,195px)}} #eOrKQUqjyvE13_to {animation: eOrKQUqjyvE13_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE13_to__to { 0% {transform: translate(230px,185px)} 11.222222% {transform: translate(230px,185px)} 16.777778% {transform: translate(240.909091px,195px)} 72.333333% {transform: translate(460.431893px,195px)} 100% {transform: translate(460.431893px,195px)}} #eOrKQUqjyvE14_to {animation: eOrKQUqjyvE14_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE14_to__to { 0% {transform: translate(340px,195px)} 11.222222% {transform: translate(340px,195px)} 72.333333% {transform: translate(580.863784px,195px)} 100% {transform: translate(580.863784px,195px)}} #eOrKQUqjyvE40_to {animation: eOrKQUqjyvE40_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE40_to__to { 0% {transform: translate(205px,110px)} 11.222222% {transform: translate(205px,110px)} 59% {transform: translate(300px,195px)} 61.222222% {transform: translate(305px,195px)} 100% {transform: translate(305px,195px)}} #eOrKQUqjyvE41_to {animation: eOrKQUqjyvE41_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE41_to__to { 0% {transform: translate(200px,100px)} 11.222222% {transform: translate(200px,100px)} 64.555556% {transform: translate(305.727273px,195px)} 66.777778% {transform: translate(315px,195px)} 100% {transform: translate(315px,195px)}} #eOrKQUqjyvE42_to {animation: eOrKQUqjyvE42_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE42_to__to { 0% {transform: translate(205px,90px)} 11.222222% {transform: translate(205px,90px)} 70.111111% {transform: translate(320.636364px,194.333333px)} 72.333333% {transform: translate(325px,195px)} 100% {transform: translate(325px,195px)}} #eOrKQUqjyvE43_to {animation: eOrKQUqjyvE43_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE43_to__to { 0% {transform: translate(222.5px,45px)} 11.222222% {transform: translate(222.5px,45px)} 72.333333% {transform: translate(342.5px,155px)} 100% {transform: translate(342.5px,155px)}} #eOrKQUqjyvE68_to {animation: eOrKQUqjyvE68_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE68_to__to { 0% {transform: translate(240px,90px)} 11.222222% {transform: translate(240px,90px)} 69% {transform: translate(353.454545px,194px)} 72.333333% {transform: translate(360px,195px)} 100% {transform: translate(360px,195px)}} #eOrKQUqjyvE69_to {animation: eOrKQUqjyvE69_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE69_to__to { 0% {transform: translate(245px,100px)} 11.222222% {transform: translate(245px,100px)} 63.444444% {transform: translate(347.545454px,194px)} 72.333333% {transform: translate(370px,195px)} 100% {transform: translate(370px,195px)}} #eOrKQUqjyvE70_to {animation: eOrKQUqjyvE70_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE70_to__to { 0% {transform: translate(240px,110px)} 11.222222% {transform: translate(240px,110px)} 59% {transform: translate(333.818181px,195px)} 72.333333% {transform: translate(380px,195px)} 100% {transform: translate(380px,195px)}} #eOrKQUqjyvE96_to {animation: eOrKQUqjyvE96_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE96_to__to { 0% {transform: translate(215px,175px)} 11.222222% {transform: translate(215px,175px)} 21.111111% {transform: translate(234.208633px,192.8px)} 22.333333% {transform: translate(235px,195px)} 100% {transform: translate(235px,195px)}} #eOrKQUqjyvE97_to {animation: eOrKQUqjyvE97_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE97_to__to { 0% {transform: translate(215px,165px)} 11.222222% {transform: translate(215px,165px)} 26.666667% {transform: translate(245px,192.8px)} 27.888889% {transform: translate(245px,195px)} 100% {transform: translate(245px,195px)}} #eOrKQUqjyvE98_to {animation: eOrKQUqjyvE98_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE98_to__to { 0% {transform: translate(215px,155px)} 11.222222% {transform: translate(215px,155px)} 31.111111% {transform: translate(254.054545px,190.8px)} 33.444444% {transform: translate(255px,195px)} 100% {transform: translate(255px,195px)}} #eOrKQUqjyvE99_to {animation: eOrKQUqjyvE99_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE99_to__to { 0% {transform: translate(215px,145px)} 11.222222% {transform: translate(215px,145px)} 36.666667% {transform: translate(264.963636px,190.8px)} 39% {transform: translate(265px,195px)} 100% {transform: translate(265px,195px)}} #eOrKQUqjyvE100_to {animation: eOrKQUqjyvE100_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE100_to__to { 0% {transform: translate(215px,135px)} 11.222222% {transform: translate(215px,135px)} 41.111111% {transform: translate(273.690909px,188.8px)} 44.555556% {transform: translate(275px,195px)} 100% {transform: translate(275px,195px)}} #eOrKQUqjyvE101_to {animation: eOrKQUqjyvE101_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE101_to__to { 0% {transform: translate(215px,125px)} 11.222222% {transform: translate(215px,125px)} 46.666667% {transform: translate(284.6px,188.8px)} 50.111111% {transform: translate(285px,195px)} 100% {transform: translate(285px,195px)}} #eOrKQUqjyvE102_to {animation: eOrKQUqjyvE102_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE102_to__to { 0% {transform: translate(215px,115px)} 11.222222% {transform: translate(215px,115px)} 50% {transform: translate(291.145455px,184.8px)} 55.666667% {transform: translate(295px,195px)} 100% {transform: translate(295px,195px)}} #eOrKQUqjyvE103_to {animation: eOrKQUqjyvE103_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE103_to__to { 0% {transform: translate(215px,185px)} 11.222222% {transform: translate(215px,185px)} 16.111111% {transform: translate(224.496403px,193.8px)} 16.777778% {transform: translate(225px,195px)} 100% {transform: translate(225px,195px)}} #eOrKQUqjyvE104_to {animation: eOrKQUqjyvE104_to__to 9000ms linear infinite normal forwards}@keyframes eOrKQUqjyvE104_to__to { 0% {transform: translate(150px,220px);animation-timing-function: cubic-bezier(0,0,0.58,1)} 11.111111% {transform: translate(150px,207px)} 100% {transform: translate(150px,207px)}}
+]]></style>
+<g><g><g><g id="eOrKQUqjyvE6_to" transform="translate(230,115)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE7_to" transform="translate(230,125)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE8_to" transform="translate(230,135)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE9_to" transform="translate(230,145)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE10_to" transform="translate(230,155)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE11_to" transform="translate(230,165)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE12_to" transform="translate(230,175)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE13_to" transform="translate(230,185)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g></g><g id="eOrKQUqjyvE14_to" transform="translate(340,195)"><g transform="translate(-340,-195)"><circle r="5" transform="translate(230 195)" fill="#482564"/><circle r="5" transform="translate(240 195)" fill="#482564"/><circle r="5" transform="translate(250 195)" fill="#482564"/><circle r="5" transform="translate(260 195)" fill="#482564"/><circle r="5" transform="translate(270 195)" fill="#482564"/><circle r="5" transform="translate(280 195)" fill="#482564"/><circle r="5" transform="translate(290 195)" fill="#482564"/><circle r="5" transform="translate(300 195)" fill="#482564"/><circle r="5" transform="translate(310 195)" fill="#482564"/><circle r="5" transform="translate(320 195)" fill="#482564"/><circle r="5" transform="translate(330 195)" fill="#482564"/><circle r="5" transform="translate(340 195)" fill="#482564"/><circle r="5" transform="translate(350 195)" fill="#482564"/><circle r="5" transform="translate(360 195)" fill="#482564"/><circle r="5" transform="translate(370 195)" fill="#482564"/><circle r="5" transform="translate(380 195)" fill="#482564"/><circle r="5" transform="translate(390 195)" fill="#482564"/><circle r="5" transform="translate(400 195)" fill="#482564"/><circle r="5" transform="translate(410 195)" fill="#482564"/><circle r="5" transform="translate(420 195)" fill="#482564"/><circle r="5" transform="translate(430 195)" fill="#482564"/><circle r="5" transform="translate(440 195)" fill="#482564"/><circle r="5" transform="translate(450 195)" fill="#482564"/></g></g></g><g><g><g id="eOrKQUqjyvE40_to" transform="translate(205,110)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE41_to" transform="translate(200,100)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g><g id="eOrKQUqjyvE42_to" transform="translate(205,90)"><circle r="5" transform="translate(0,0)" fill="#482564"/></g></g><g id="eOrKQUqjyvE43_to" transform="translate(222.5,45)"><g transform="translate(-222.5,-45)"><g><circle r="5" transform="translate(205 40)" fill="#482564"/><circle r="5" transform="translate(200 30)" fill="#482564"/><circle r="5" transform="translate(201 19)" fill="#482564"/><g><circle r="5" transform="translate(207 10)" fill="#838383"/><circle r="5" transform="translate(217 5)" fill="#838383"/><circle r="5" transform="translate(228 5)" fill="#838383"/><circle r="5" transform="translate(238 10)" fill="#838383"/><circle r="5" transform="translate(244 19)" fill="#838383"/><circle r="5" transform="translate(245 30)" fill="#838383"/><circle r="5" transform="translate(240 40)" fill="#838383"/></g></g><g><circle r="5" transform="translate(215 85)" fill="#482564"/><circle r="5" transform="translate(215 75)" fill="#482564"/><circle r="5" transform="translate(215 65)" fill="#482564"/><circle r="5" transform="translate(215 55)" fill="#482564"/><circle r="5" transform="translate(215 45)" fill="#482564"/><circle r="5" transform="translate(230 55)" fill="#482564"/><circle r="5" transform="translate(230 45)" fill="#482564"/><circle r="5" transform="translate(230 65)" fill="#482564"/><circle r="5" transform="translate(230 75)" fill="#482564"/><circle r="5" transform="translate(230 85)" fill="#482564"/></g></g></g><g><g id="eOrKQUqjyvE68_to" transform="translate(240,90)"><circle r="5" transform="translate(0,0)" fill="#7f6494"/></g><g id="eOrKQUqjyvE69_to" transform="translate(245,100)"><circle r="5" transform="translate(0,0)" fill="#7f6494"/></g><g id="eOrKQUqjyvE70_to" transform="translate(240,110)"><circle r="5" transform="translate(0,0)" fill="#7f6494"/></g></g></g><g><g><circle r="5" transform="translate(15 195)" fill="#7acbbc"/><circle r="5" transform="translate(35 195)" fill="#7acbbc"/><circle r="5" transform="translate(55 195)" fill="#7acbbc"/><circle r="5" transform="translate(75 195)" fill="#7acbbc"/><circle r="5" transform="translate(95 195)" fill="#7acbbc"/><circle r="5" transform="translate(115 195)" fill="#7acbbc"/><circle r="5" transform="translate(135 195)" fill="#7acbbc"/><circle r="5" transform="translate(155 195)" fill="#7acbbc"/><circle r="5" transform="translate(175 195)" fill="#7acbbc"/><circle r="5" transform="translate(195 195)" fill="#7acbbc"/><circle r="5" transform="translate(215 195)" fill="#7acbbc"/><circle r="5" transform="translate(5 195)" fill="#7acbbc"/><circle r="5" transform="translate(25 195)" fill="#7acbbc"/><circle r="5" transform="translate(45 195)" fill="#7acbbc"/><circle r="5" transform="translate(65 195)" fill="#7acbbc"/><circle r="5" transform="translate(85 195)" fill="#7acbbc"/><circle r="5" transform="translate(105 195)" fill="#7acbbc"/><circle r="5" transform="translate(125 195)" fill="#7acbbc"/><circle r="5" transform="translate(145 195)" fill="#7acbbc"/><circle r="5" transform="translate(165 195)" fill="#7acbbc"/><circle r="5" transform="translate(185 195)" fill="#7acbbc"/><circle r="5" transform="translate(205 195)" fill="#7acbbc"/><g><g id="eOrKQUqjyvE96_to" transform="translate(215,175)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE97_to" transform="translate(215,165)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE98_to" transform="translate(215,155)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE99_to" transform="translate(215,145)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE100_to" transform="translate(215,135)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE101_to" transform="translate(215,125)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE102_to" transform="translate(215,115)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g><g id="eOrKQUqjyvE103_to" transform="translate(215,185)"><circle r="5" transform="translate(0,0)" fill="#7acbbc"/></g></g></g></g><g id="eOrKQUqjyvE104_to" transform="translate(150,220)"><g transform="translate(-150,-210)"><circle r="5" transform="translate(15 210)" fill="#1f8875"/><circle r="5" transform="translate(35 210)" fill="#1f8875"/><circle r="5" transform="translate(55 210)" fill="#1f8875"/><circle r="5" transform="translate(75 210)" fill="#1f8875"/><circle r="5" transform="translate(95 210)" fill="#1f8875"/><circle r="5" transform="translate(115 210)" fill="#1f8875"/><circle r="5" transform="translate(135 210)" fill="#1f8875"/><circle r="5" transform="translate(155 210)" fill="#1f8875"/><circle r="5" transform="translate(175 210)" fill="#1f8875"/><circle r="5" transform="translate(195 210)" fill="#1f8875"/><circle r="5" transform="translate(215 210)" fill="#1f8875"/><circle r="5" transform="translate(225 210)" fill="#1f8875"/><circle r="5" transform="translate(235 210)" fill="#1f8875"/><circle r="5" transform="translate(245 210)" fill="#1f8875"/><circle r="5" transform="translate(255 210)" fill="#1f8875"/><circle r="5" transform="translate(265 210)" fill="#1f8875"/><circle r="5" transform="translate(275 210)" fill="#1f8875"/><circle r="5" transform="translate(285 210)" fill="#1f8875"/><circle r="5" transform="translate(295 210)" fill="#1f8875"/><circle r="5" transform="translate(5 210)" fill="#1f8875"/><circle r="5" transform="translate(25 210)" fill="#1f8875"/><circle r="5" transform="translate(45 210)" fill="#1f8875"/><circle r="5" transform="translate(65 210)" fill="#1f8875"/><circle r="5" transform="translate(85 210)" fill="#1f8875"/><circle r="5" transform="translate(105 210)" fill="#1f8875"/><circle r="5" transform="translate(125 210)" fill="#1f8875"/><circle r="5" transform="translate(145 210)" fill="#1f8875"/><circle r="5" transform="translate(165 210)" fill="#1f8875"/><circle r="5" transform="translate(185 210)" fill="#1f8875"/><circle r="5" transform="translate(205 210)" fill="#1f8875"/></g></g></g></svg>
+</div>
+</center>
+</html>
 <!-- -->
@@ Line 12: / Line 22: @@
 <partinfo>BBa_K4207023 SequenceAndFeatures</partinfo>
+===2. Design===
+===3. Characterization===
+<gallery caption="Expected structures" widths="400px" heights="400px" perrow="6">
+File:PMV_A2_inactive.png|Structure of inactive form
+File:PMV_A2_active.png|Structure of active form
+</gallery>
+===4. Conclusion===
+===5. References===
 <!-- Uncomment this to enable Functional Parameter display