Difference between revisions of "Part:BBa K239005:Design"

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Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP.  
 
Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP.  
 
J Bacteriol. 1992 Nov;174(22):7104-11.
 
J Bacteriol. 1992 Nov;174(22):7104-11.
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Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901

Revision as of 11:23, 16 September 2009

NarK promoter, Fnr activated under anaerobic conditions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the IHF binding site and ends 6 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP. J Bacteriol. 1992 Nov;174(22):7104-11.


Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901