Difference between revisions of "Part:BBa K4368005"
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It is a composition based on the ''cI'' repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (<partinfo>BBa_C0051</partinfo>). In the case of our team, it has been placed under the control of the pcstA promoter (<partinfo>BBa_K118011</partinfo>), together with a strong rbs (<partinfo>BBa_B0030</partinfo>) and double terminator (<partinfo>BBa_B0015</partinfo>). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria. | It is a composition based on the ''cI'' repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (<partinfo>BBa_C0051</partinfo>). In the case of our team, it has been placed under the control of the pcstA promoter (<partinfo>BBa_K118011</partinfo>), together with a strong rbs (<partinfo>BBa_B0030</partinfo>) and double terminator (<partinfo>BBa_B0015</partinfo>). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria. | ||
| + | ==Characterization== | ||
| + | xxx | ||
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Revision as of 14:51, 6 October 2022
pcstA + rbs + cI + terminator
Description
It is a composition based on the cI repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (BBa_C0051). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (BBa_B0030) and double terminator (BBa_B0015). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.
Characterization
xxx
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Contribution
- Group: [1]
- Author: Jiménez Amores, Carmen