Difference between revisions of "Part:BBa K4385011:Design"
(→‎Source)
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
It is amplified from E. coli genomic DNA.
+
It comes from the part BBa_K4385009 and BBa_E0040
  
 
===References===
 
===References===

Latest revision as of 14:16, 6 October 2022


Lead sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394


Design Notes

The lead sensor is composed of BBa_K4385009 pbr and BBa_E0040 GFP. Note the order of inducible promoter Pbc, constitutive promoter Pbr and lead-sensing regulatory protein PbrR in pbr.

Source

It comes from the part BBa_K4385009 and BBa_E0040

References