Difference between revisions of "Part:BBa K4165080"
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9.232 kDa
 
9.232 kDa
  
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It has both x-ray structure and a predicted model (AlphaFold).
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===PDB Structure===
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PDB structure
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It has both NMR structure and a predicted model (AlphaFold).
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NMR structure:  
 
NMR structure:  
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C-beta Deviations:  
 
C-beta Deviations:  
 
Q-Mean:  
 
Q-Mean:  
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/p85062-nmr.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: A graphical illustration showing the Inhibitor (NMR).
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AlphaFold:
 
AlphaFold:
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C-beta Deviations:  
 
C-beta Deviations:  
 
Q-Mean:  
 
Q-Mean:  
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/p85062-alphafold.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: A graphical illustration showing the Inhibitor (AlphaFold).
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===References===
 
===References===

Revision as of 13:57, 6 October 2022


SPINK7 (Serine Peptidase Inhibitor Kazal type 7).

This basic part encodes Human serine protease inhibitor known as SPINK7 which is able to inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).


Usage and Biology

This type of family encodes for a type of inhibitor that is able to inhibit serine proteases. The inhibitor binds to trypsin proteases and since the catalytic core of HtrA1 (BBa_K4165004) is considered as a tyrpsin-like catalytic domain, so this inhibitor also is considered to inhibit the function of HtrA1 [1] - [4].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GC% Content 53.3% Isoelectric point (PI) 7.268 Charge at pH 7 0.566 Molecular Weight (Protein) 9.232 kDa

PDB Structure

It has both NMR structure and a predicted model (AlphaFold).


NMR structure: https://www.rcsb.org/structure/2LEO Molprobity: Clash Score: Ramachandran Favoured: Ramachandran Outliers: Rotamers Outliers: C-beta Deviations: Q-Mean: 


                 Figure 1.: A graphical illustration showing the Inhibitor (NMR).


AlphaFold: https://alphafold.ebi.ac.uk/entry/P58062 Molprobity: Clash Score: Ramachandran Favoured: Ramachandran Outliers: Rotamers Outliers: C-beta Deviations: Q-Mean: 


                 Figure 1.: A graphical illustration showing the Inhibitor (AlphaFold).


References

1 - Frochaux, V., Hildebrand, D., Talke, A., Linscheid, M. W., & Schlüter, H. (2014). Alpha-1-antitrypsin: a novel human high temperature requirement protease A1 (HTRA1) substrate in human placental tissue. PloS one, 9(10), e109483. 2 - Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026. 3 - Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050. 4 - Chen, T. J., Tian, Y. F., Chou, C. L., Chan, T. C., He, H. L., Li, W. S., ... & Lai, H. Y. (2021). High spink4 expression predicts poor outcomes among rectal cancer patients receiving CCRT. Current Oncology, 28(4), 2373-2384.