Difference between revisions of "Part:BBa K4361000"

Revision as of 13:51, 6 October 2022

BlcR-binding oligo, 51 bp, scrambled

Firstly, the wildtype sequence was scrambled using the Genscript Sequence Scramble tool (https://www.genscript.com/tools/create-scrambled-sequence) to create an oligo containing the same nucleotides in a randomized order. Since this oligo does not contain either of the inverted repeat pairs, BlcR should not be able to specifically bind this molecule, thus it can be used as a negative control to compare other sequences to. This oligo was designated BBa_K4361000.

Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 37
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 37
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4361000 short</partinfo>
+Firstly, the wildtype sequence was scrambled using the Genscript Sequence Scramble tool (https://www.genscript.com/tools/create-scrambled-sequence) to create an oligo containing the same nucleotides in a randomized order. Since this oligo does not contain either of the inverted repeat pairs, BlcR should not be able to specifically bind this molecule, thus it can be used as a negative control to compare other sequences to. This oligo was designated BBa_K4361000.
 Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===