Difference between revisions of "Part:BBa K4385011"

 
Line 3: Line 3:
 
<partinfo>BBa_K4385011 short</partinfo>
 
<partinfo>BBa_K4385011 short</partinfo>
  
−
This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC  promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
+
A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream GFP (green fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:41, 6 October 2022


Lead sensor

A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream GFP (green fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394