Difference between revisions of "Part:BBa J70500:Design"
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Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly. | Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly. | ||
| + | * PCR with primers: | ||
| + | ** WV-F: gtttcttcgaattcgcggccgcttctagagcgattttttattaaaacgtctcaaaatcgtttctg | ||
| + | ** WV-R: gtttcttcctgcagcggccgctactagtaatcattttgtttattgcaattgtattgctattaatcg | ||
| − | + | These primers were designed with Austin's GBW tool. | |
===Source=== | ===Source=== | ||
Revision as of 22:15, 13 September 2009
pWV01 broad host range plasmid origin and rep protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly.
- PCR with primers:
- WV-F: gtttcttcgaattcgcggccgcttctagagcgattttttattaaaacgtctcaaaatcgtttctg
- WV-R: gtttcttcctgcagcggccgctactagtaatcattttgtttattgcaattgtattgctattaatcg
These primers were designed with Austin's GBW tool.
Source
PCR of the plasmid pMG, kind gift from Prof. Jan Kok. This plasmid contains the ClaI fragment of the pWV01 plasmid.