Difference between revisions of "Part:BBa K4425002"
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<partinfo>BBa_K4425002 short</partinfo> | <partinfo>BBa_K4425002 short</partinfo> | ||
− | a glucosyltransferase | + | a glucosyltransferase that can introduce a reversible protecting glucose group into indoxyl, turning low water solubility product indigo (or tyrian purple) to soluble indican. |
+ | [[Image:25001-2.png|thumbnail|200px|center|'''Figure 1:''' PtUGT can convert indoxyl to indican ]] | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | gene of PtUGT and FMO was assembled with pET28a backbone to construct pET28a-PtUGT-FMO. PtUGT and FMO were co-expression to convert indole (or 6-Br-indole) to indican which gives a sustainable indigo dyeing strategy. | ||
+ | [[Image:25002-1.png|thumbnail|400px|center|'''Figure 2:''' schematic of pET28a-PtUGT-FMO ]] | ||
+ | |||
+ | ==Source== | ||
+ | PtUGT come from <i>P. tinctorium</i> | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4425002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4425002 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K4425002 parameters</partinfo> | <partinfo>BBa_K4425002 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | ==Reference== | ||
+ | Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261. |
Latest revision as of 09:07, 6 October 2022
PtUGT
a glucosyltransferase that can introduce a reversible protecting glucose group into indoxyl, turning low water solubility product indigo (or tyrian purple) to soluble indican.
Usage and Biology
gene of PtUGT and FMO was assembled with pET28a backbone to construct pET28a-PtUGT-FMO. PtUGT and FMO were co-expression to convert indole (or 6-Br-indole) to indican which gives a sustainable indigo dyeing strategy.
Source
PtUGT come from P. tinctorium
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 886
Illegal AgeI site found at 1093 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 484
Reference
Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.