Difference between revisions of "Part:BBa K4162005"

Revision as of 08:22, 6 October 2022

Hammerhead ribozyme

Introduction

Hammerhead ribozyme was first found in the genome of viruses and viroids. It involved in the processing of RNA transcripts based on rolling-circle replication. The tandem copy of RNA sequence will be generated in the roll ring replication, and the self-cleaving activity of ribozyme can ensure the generation of RNA copy of unit length.^[1]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ Ferré-D'Amaré, Adrian R, and William G Scott. “Small self-cleaving ribozymes.” Cold Spring Harbor perspectives in biology vol. 2,10 (2010): a003574. doi:10.1101/cshperspect.a003574

[1] Ferré-D'Amaré, Adrian R, and William G Scott. “Small self-cleaving ribozymes.” Cold Spring Harbor perspectives in biology vol. 2,10 (2010): a003574. doi:10.1101/cshperspect.a003574

[1]

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 <partinfo>BBa_K4162005 short</partinfo>
-Ribozymes are non-coding RNAs that can catalyze specific biochemical reactions without the assistance of proteins, involved in a variety of vital cellular reactions. Ribozymes can be divided into RNA metalloenzymes and self-cleaving ribozymes. Studies have shown that self-cleaving ribozymes adopt a general acid-base catalytic mechanism to perform site-specific phosphodiester bond cleavage. Until now, only ten families of self-cleaving ribozymes have been discovered, and hammerhead ribozyme is one of them. By inserting the DNA sequence of hammerhead ribozyme between two coding sequences, mRNA transcribed by this gene will perform self cleavage at the ribozyme site, so that a long piece of mRNA will be cut into segments to prevent the translation process of various coding sequences from interfering with each other.<ref>Blount, K F, and O C Uhlenbeck. “The hammerhead ribozyme.” Biochemical Society transactions vol. 30,Pt 6 (2002): 1119-22. doi:10.1042/bst0301119</ref>
+===Introduction===
+Hammerhead ribozyme was first found in the genome of viruses and viroids. It involved in the processing of RNA transcripts based on rolling-circle replication. The tandem copy of RNA sequence will be generated in the roll ring replication, and the self-cleaving activity of ribozyme can ensure the generation of RNA copy of unit length.<ref>Ferré-D'Amaré, Adrian R, and William G Scott. “Small self-cleaving ribozymes.” Cold Spring Harbor perspectives in biology vol. 2,10 (2010): a003574. doi:10.1101/cshperspect.a003574</ref>
+[[File:T--Fudan--rolling-circle replication.jpg|'''Figure 1. Self-cleaving ribozymes play a role in rolling-circle replication of some Viral and viroid genomes.''']]