Difference between revisions of "Part:BBa K4368001"

Revision as of 08:06, 6 October 2022

pcstA + rbs + yebF + cex + terminator

CenA encodes for the endoglucanase gene of Celullomonas fimi (BBa_K118022). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1044
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1552
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1097
Illegal SapI.rc site found at 1180

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4368001 short</partinfo>
-Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.
+''CenA'' encodes for the endoglucanase gene of ''Celullomonas fimi'' (<partinfo>BBa_K118022</partinfo>). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by the glucose concentration (<partinfo>BBA_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane.
+The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
 <!-- Add more about the biology of this part here