Difference between revisions of "Part:BBa K4368000"
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<partinfo>BBa_K4368000 short</partinfo> | <partinfo>BBa_K4368000 short</partinfo> | ||
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''CenA'' encodes for the endoglucanase gene of ''Celullomonas fimi'' (<partinfo>BBa_K118023</partinfo>). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by the glucose concentration (<partinfo>BBA_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane. | ''CenA'' encodes for the endoglucanase gene of ''Celullomonas fimi'' (<partinfo>BBa_K118023</partinfo>). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by the glucose concentration (<partinfo>BBA_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane. | ||
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts. | The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts. | ||
Revision as of 08:05, 6 October 2022
pcstA + rbs + yebF + cenA + terminator
CenA encodes for the endoglucanase gene of Celullomonas fimi (BBa_K118023). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 554
Illegal NotI site found at 1698 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BglII site found at 1611
Illegal BamHI site found at 747
Illegal XhoI site found at 1109
Illegal XhoI site found at 1358 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 889
Illegal NgoMIV site found at 1814 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 793