Difference between revisions of "Part:BBa K4323002"
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<partinfo>BBa_K4323002 short</partinfo> | <partinfo>BBa_K4323002 short</partinfo> | ||
| − | This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006. | + | This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_BBa_K2683006 BBa_K2683006]. |
[[File:FitD toxicity.png|800px|center|FitD toxicity]] | [[File:FitD toxicity.png|800px|center|FitD toxicity]] | ||
Revision as of 00:06, 6 October 2022
Truncated FitD
This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.
Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 909
Illegal BamHI site found at 1019
Illegal BamHI site found at 2568 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2191
- 1000COMPATIBLE WITH RFC[1000]